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acuA [2017-11-03 09:08:19]
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acuA [2017-11-03 09:08:19]

protein acetylase for the control of AcsA activity
locus
BSU29690
pI
5.03
mw
24.18 kDa
protein length
210 aa Sequence Blast
gene length
630 bp Sequence Blast
function
control of AcsA activity
product
Gcn5-related N-acetyltransferase
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.1|Utilization of organic acids]
  • [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.1|Utilization of lipids] → [category|SW 2.4.1.3|Utilization of fatty acids]
  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.5|Protein acetylases/ deacetylases]

    • Gene

    Coordinates
    3,040,092 → 3,040,724

    The protein

    Catalyzed reaction/ biological activity

  • acetylates (and thereby inactivates) [protein|6671E7D85E99D349679F0E9D825DC035D10FFD2E|AcsA] on Lys-549 [Pubmed|18487328] [Pubmed|19136592]

    • Protein family

  • acetyltransferase family (according to Swiss-Prot) Gcn5-relatedN-acetyltransferase [Pubmed|18487328]

    • Expression and Regulation

    Operons

    genes
    [gene|17C3F56CE859BCD7A282C144C46CC92D3506ABF6|acuA]-[gene|FC9981CFB9D7EB8ACDA3032EC2F0538988F277AA|acuB]-[gene|1255813797A83D835E0656A2AAAD361B5BB2094B|acuC]
    description
    [Pubmed|7934817]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|7913927], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|7913927], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

    • regulation

  • repressed by glucose (3.4-fold) ([protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]) [Pubmed|7913927,12850135]

    • additional information

  • the mRNA is quite stable (half-life 5 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]
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    Biological materials

    Mutant

  • GP1211 (''acuA''::''kan''), available in [SW|Jörg Stülke]'s lab
  • GP1255 (''[gene|17C3F56CE859BCD7A282C144C46CC92D3506ABF6|acuA]''::''kan'' ''[gene|6072A6EF0766F3D1B1616A20CDC36CCDC6CBDA71|yjcK]''::''cat'' ''[gene|18FA5EFCD78C74296F9E52A64DA4A9D635944528|ynaD]''::''spec'' ''[gene|4A8360E50ADBA4CC859A64A72EBC0A07F70A1764|yoaA]''::''tet''), available in [SW|Jörg Stülke]'s lab
  • BKE29690 (Δ[gene|17C3F56CE859BCD7A282C144C46CC92D3506ABF6|acuA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29690 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTAATTCACCGTCCCATC, downstream forward: _UP4_TAACTGACACTGACAAAGGG
  • BKK29690 (Δ[gene|17C3F56CE859BCD7A282C144C46CC92D3506ABF6|acuA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29690 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTAATTCACCGTCCCATC, downstream forward: _UP4_TAACTGACACTGACAAAGGG

    • References

  • 7913927,22900538,16855235,7934817,19136592,18487328,12850135,26098117